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Merck

85578

Spermidine trihydrochloride

BioXtra, ≥99.5% (AT)

Synonym(s):

N-(3-Aminopropyl)-1,4-butanediamine trihydrochloride

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About This Item

Linear Formula:
NH2(CH2)3NH(CH2)4NH2 · 3HCl
CAS Number:
334-50-9
Molecular Weight:
254.63
UNSPSC Code:
12352116
NACRES:
NA.26
PubChem Substance ID:
57653005
EC Number:
206-379-0
Beilstein/REAXYS Number:
3552356
MDL number:
MFCD00012918

Key Documents

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Product Name

Spermidine trihydrochloride, BioXtra, ≥99.5% (AT)

product line

BioXtra

assay

≥99.0% (TLC)
≥99.5% (AT)

form

powder

impurities

insoluble matter, passes filter test

pH

3.0-6.0 (25 °C, 1 M in H2O)

mp

257-259 °C (lit.)

InChI key

LCNBIHVSOPXFMR-UHFFFAOYSA-N

InChI

1S/C7H19N3.3ClH/c8-4-1-2-6-10-7-3-5-9;;;/h10H,1-9H2;3*1H

SMILES string

Cl[H].Cl[H].Cl[H].NCCCCNCCCN

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤10 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.2
λ: 280 nm Amax: 0.1

Quality Level

100

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Application

Spermidine trihydrochloride is used for the following applications:
  • DNA extraction and restriction endonuclease digestion
  • Nuclear transplantation
  • During the chemical analyses of the squid species Todaropsis eblanae
  • As one of the agents in intercalating drug.
  • Platelet aggregation
It may be used in the synthesis of palladium complexes like [sperH3]2[PdCl4]3, [PdCl2(sperH)]2[PdCl4], and [(PdCl2)3(sper)2] by reacting with K2PdCl4 and PdCl2.

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T4 polynucleotide kinase activity.
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.

Disclaimer

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

General description

Spermidine trihydrochloride is an aliphatic polyamine. Its crystalline structure has been studied. It has been reported to preserve the nuclei and prevent proteolytic degradation of histones during the macronuclei isolation from Tetrahymena pyriformis.

Other Notes

Buffer component for chromosome isolation in bivariate flow cytogenetic analysis and sorting.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN2778
BCCN2777
BCCK0100
BCCL0203
BCCL0202

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M A Gorovsky et al.
The Journal of cell biology, 61(1), 134-145 (1974-04-01)
Histone fraction F1 has been isolated and purified from macronuclei of the ciliated protozoan, Tetrahymena pyriformis. In many respects, Tetrahymena F1 is similar to that of other organisms. It is the only Tetrahymena histone soluble in 5% perchloric acid or
J R Gibson et al.
Journal of clinical microbiology, 36(9), 2580-2585 (1998-08-15)
Amplified-fragment length polymorphism (AFLP) analysis is the name given to a genotypic technique in which adapter oligonucleotides are ligated to restriction enzyme fragments and then used as target sites for primers in a PCR amplification process. The amplified fragments are
C Navarro-Ranninger et al.
Journal of inorganic biochemistry, 46(4), 267-279 (1992-06-01)
By reaction of spermidine trihydrochloride with K2PdCl4 and PdCl2 at different pH's, we have synthesized the [sperH3]2[PdCl4]3 (I), [PdCl2(sperH)]2[PdCl4] (II), and [(PdCl2)3(sper)2] (III) compounds. The structure of these compounds was studied by IR and 1H NMR; complex II was analyzed
J J Welch et al.
The Journal of biological chemistry, 269(49), 31051-31058 (1994-12-09)
Intercalating, minor groove binding, and covalently bonding drugs were evaluated by mobility shift assays for their ability to interfere with transcription factors binding to their respective DNA recognition sequences. The Cys2His2 zinc finger proteins EGR1, WT1, and NIL2A, the basic
T Paarup et al.
Journal of applied microbiology, 92(5), 941-950 (2002-04-26)
To relate sensory shelf-life of iced whole and gutted squid to bacterial growth and chemical changes. Cooked mantles from whole and gutted individuals were rejected after 10 and 12 days of storage, respectively, due to ammoniacal off-odours. Rate of production
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