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Merck

A1888

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

≥98% (HPLC), chromogenic, powder

Synonym(s):

AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

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About This Item

Empirical Formula (Hill Notation):
C18H24N6O6S4
CAS Number:
30931-67-0
Molecular Weight:
548.68
NACRES:
NA.83
PubChem Substance ID:
24890599
UNSPSC Code:
12352204
EC Number:
250-396-6
MDL number:
MFCD00010404
Beilstein/REAXYS Number:
7329461

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Product Name

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)

InChI key

OHDRQQURAXLVGJ-AXMZSLBLSA-N

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

SMILES string

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

assay

≥98% (HPLC)

form

powder

solubility

water: 10 mg/mL, clear to slightly hazy

storage temp.

2-8°C

Quality Level

200

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Application

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
  • 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).
  • ABTS is significantly used by the food industry, to measure the antioxidant capacities within different foods.
  • ABTS is also used to monitor glucose concentrations in different solutions including blood serum.
  • It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results.

General description

ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate:
  • ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example).
  • ABTS together with laccase or bilirubin oxidase.

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).††† ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000523637
0000523637
0000521680
0000521680
0000515802

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Expression of glucocorticoid resistance following social stress requires a second signal.
Avitsur R et al.
Journal of Leukocyte Biology, 74, 507-507 (2003)
Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis.
Matsuda H et al.
The Japanese Journal of Experimental Medicine, 54, 131-131 (1984)
Structure-based mutagenesis of herpes simplex virus glycoprotein D defines three critical regions at the gD-HveA/HVEM binding interface.
Connolly SA et al.
Journal of Virology, 77, 8127-8127 (2003)
A simple, specific, and highly sensitive blocking enzyme-linked immunosorbent assay for detection of antibodies to bovine herpesvirus 1.
Kramps JA et al.
Journal of Clinical Microbiology, 32, 2175-2175 (1994)
Crowther JR
ELISA: Theory and Practice (1995)
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