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A3699

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 37.5:1

Synonym(s):

30% Acrylamide/Bisacrylamide 37.5:1, Polyacrylamide solution

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About This Item

UNSPSC Code:
41105319
PubChem Substance ID:
329770843
NACRES:
NA.25
MDL number:
MFCD00080848
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Product Name

Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 37.5:1

sterility

0.2 μm filtered

Quality Level

100

product line

BioReagent

form

liquid

feed ratio

37.5:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

storage temp.

2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
Acrylamide/Bis-acrylamide, 30% solution has been used:
  • in the preparation of polyacrylamide gel for photobleaching experiment
  • as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution
  • in the preparation of polyacrylamide gels during western blot analyses

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

79-06-1

CAS No.

79-06-1

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
0000532912
0000512673
0000512673
0000532912
0000481784

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Related Content

Data Sheet - A3699

Instructions

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Single cell--resolution western blotting
Kang CC, et al.
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