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Merck

B3133

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

trypsin substrate, chromogenic, ≥99% (TLC), powder, suitable for substrate for trypsin

Synonym(s):

L-BAPA, L-BAPNA, BANI

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About This Item

Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
Molecular Weight:
434.88
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
244-505-6
MDL number:
Beilstein/REAXYS Number:
4081878
NACRES:
NA.32
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Product Name

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride, ≥99% (TLC), suitable for substrate for trypsin

InChI key

DEOKFPFLXFNAON-NTISSMGPSA-N

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

SMILES string

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

assay

≥99% (TLC)

form

powder

solubility

acetone: water (1:1): 50 mg/mL

suitability

suitable for substrate for trypsin

storage temp.

−20°C

Quality Level

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Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as a substrate:
  • for chymotrypsin and trypsin activity assay in enzymatic extract (EE) of M. tenellum and pancreatic tissue homogenates
  • in trypsin and papain standard curve generation
  • in serine protease and fibrinogenolytic assays of eupolytin1 protein

General description

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride is a substrate for trypsin.
Chromogenic substrate for trypsin and other proteolytic enzymes.

Other Notes

Similar to B3279, but produced for Sigma.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Robert L Pizzey et al.
International dental journal, 61 Suppl 3, 33-40 (2011-11-02)
This study aimed to evaluate antimicrobial effects of an o-cymen-5-ol/zinc system. o-Cymen-5-ol and zinc gluconate minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined against Streptococcus mutans, Actinomyces viscosus, Porphyromonas gingivalis, Fusobacterium nucleatum and Candida albicans. Synergy was
In vitro protein digestibility of animal, vegetal and microbial feed ingredients for Macrobrachium tenellum
Nolasco-Soria H, et al.
Latin American Journal of Aquatic Research, 46(3), 157-162 (2018)
Enzymatic Assays and Enzyme Histochemistry of Tuta absoluta Feeding on Tomato Leaves
Hamza R, et al.
BMC plant biology, 8(17), 1256-1263 (2018)
Feeding colostrum, its composition and feeding duration variably modify proliferation and morphology of the intestine and digestive enzyme activities of neonatal calves
Blattler U, et al.
The Journal of Nutrition, 131(4), 1256-1263 (2001)
The action of trypsin on synthetic chromogenic arginine substrates
Somorin O, et al.
Journal of Biochemistry, 85(1), 157-162 (1979)

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