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About This Item
UNSPSC Code:
41106514
Biological source:
human skin (lymph node metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
human skin (lymph node metastasis)
packaging
tube of 5 μg 91100402-DNA-5UG, pkg of vial of cells 91100402-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Nerve growth factor (NGF), Laminin
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human caucasian metastatic melanoma
This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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