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Merck

F1804

ANTI-FLAG® M2 antibody, Mouse monoclonal

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonym(s):

flag antibody, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
M2, monoclonal
Application:
—
Species reactivity:
all
Citations:
9676

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Related Categories

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000528190
0000528186
0000528190
0000528186
0000528189

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Monika Srivastava et al.
Nature communications, 6, 6253-6253 (2015-02-24)
Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquin(san)), Icos is overexpressed in T cells. Here we show that Roquin enhances
Histone H1 Limits DNA Methylation in Neurospora crassa
Michael Seymour
G3 (Bethesda, Md.) (2016)
HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway
Yalan Liu
Virology, 154-154 (2016)
ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins
Aparna A
Nucleic Acids Research, 45(7), 4189-4201 (2017)
Yumin Qiu et al.
Biochimica et biophysica acta. Molecular cell research, 1866(9), 1412-1420 (2019-06-10)
Src is a known proto-oncogene and its aberrant activity is involved in a variety of cancers, including ovarian cancer, whereas the regulatory mechanism of Src has not been fully clarified. In this study, we identified tripartite motif-containing (TRIM) 50 as
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