H1387
Hanks′ Balanced Salts
Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
HBSS
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About This Item
General description
Balanced salt solution (BSS) serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It also provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. BSS when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. In addition, it also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Application
Hanks′ Balanced Salts has been used:
- for diluting digested tissues during isolation of pancreatic blood vessels
- for washing mouse intestinal mucosal cells before lysis and sonication
- as an immersion solution to test the in vitro bioactivity of poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone
Preparation Note
Formulated to contain 9.7 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.
Other Notes
The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dajana Milovac et al.
Materials science & engineering. C, Materials for biological applications, 34, 437-445 (2013-11-26)
In the present study, poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone was prepared. Hydrothermal transformation of aragonitic cuttlefish bone into hydroxyapatite (HAp) was performed at 200°C retaining the cuttlebone architecture. The HAp scaffold was coated with a poly(ε-caprolactone) (PCL) using
Ehsan Mostaed et al.
Acta biomaterialia, 105, 319-335 (2020-01-27)
Zn-based alloys are recognized as promising bioabsorbable materials for cardiovascular stents, due to their biocompatibility and favorable degradability as compared to Mg. However, both low strength and intrinsic mechanical instability arising from a strong strain rate sensitivity and strain softening
Andrew I U Shearn et al.
Frontiers in cell and developmental biology, 8, 367-367 (2020-06-13)
Small extracellular vesicles (sEVs) are those nanovesicles 30-150 nm in size with a role in cell signalling and potential as biomarkers of disease. Nanoparticle tracking analysis (NTA) techniques are commonly used to measure sEV concentration in biofluids. However, this quantification