H2519

Heparinase I from Flavobacterium heparinum

Name: Heparinase I from <I>Flavobacterium heparinum</I>
Brand: SIGMA

Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)

Synonym(s):

Heparin lyase I, Heparinase

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All Photos(1)

About This Item

CAS Number:

9025-39-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

4.2.2.7 (BRENDA, IUBMB)

MDL number:

MFCD00131317

Product Name

Heparinase I from Flavobacterium heparinum, Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)

conjugate

conjugate (Glucosaminoglycan)

form

lyophilized solid

specific activity

≥200 units/mg protein (enzyme + BSA)

mol wt

42.8 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Quality Level

200

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Application

Heparinase I from Flavobacterium heparinum has been used:

in the digestion of plasma samples
to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia
in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells

Biochem/physiol Actions

Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.

Other Notes

One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000429785

0000407596

0000407595

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Cardiopulmonary Bypass: Principles and Practice, 509-509 (2008)

Thromboelastography to monitor the intra-operative effects of low-molecular weight heparin following bridging anticoagulation in a child with normal renal function*.

M E Casey et al.

Anaesthesia, 68(1), 91-96 (2012-10-16)

Paediatric patients who require anticoagulation with therapeutic doses of low-molecular weight heparin are at risk of having a residual anticoagulant effect at the time of surgery, even if managed according to current peri-operative guidelines. Testing for residual effect is not

Methodological aspects affecting the infectivity of Chlamydia pneumoniae in cell cultures in vitro

Yan Y, et al.

Journal of Microbiological Methods, 61(1), 127-130 (2005)

Crystal structure of Pedobacter heparinus heparin lyase Hep III with the active site in a deep cleft.

Wataru Hashimoto et al.

Biochemistry, 53(4), 777-786 (2014-01-21)

Pedobacter heparinus (formerly known as Flavobacterium heparinum) is a typical glycosaminoglycan-degrading bacterium that produces three heparin lyases, Hep I, Hep II, and Hep III, which act on heparins with 1,4-glycoside bonds between uronate and amino sugar residues. Being different from

HeparinHeparin - A Century of Progress, 273-273 (2012)

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