H7006
HEPES sodium salt
≥99.0% (titration)
Synonym(s):
Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
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About This Item
Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
Molecular Weight:
260.29
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
EC Number:
278-169-7
MDL number:
Quality Level
assay
≥99.0% (titration)
form
powder
storage condition
dry at room temperature
technique(s)
HPLC: suitable
color
white
pH
10-12 (1 M in water)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
water: 0.1 M, clear, colorless
suitability
suitable for mass spectrometry
application(s)
diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics
storage temp.
room temp
SMILES string
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
InChI key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
General description
HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.
Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
Application
HEPES buffer finds application in:
It has been used:
- Most studies with metal ions
- Substitute for Tris and phosphate in studies with metal ions
- Grinding buffer in plant studies
- Running buffer in gel electrophoresis
- Buffer in electroporation studies
- Bradford or bicinchoninic acid (BCA) assays
- Extraction buffer to prevent damage of proteins in red blood cells
It has been used:
- as a buffer component in the preparation of a low sucrose buffer
- as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions
Features and Benefits
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Highly soluble in water
- Negligible metal ion binding
- Less toxic to cells than other buffers such as Tris and phosphate
- Stable in a wide pH range
Packaging
The DRYPOUR®packaging system for high-quality hygroscopic salts DRYPOUR® packaging:
- The current DRYPOUR® packaging configuration is in a 50L nestable drum
- 25KG-DP are the standard prepack offering
- Smaller volumes can be put in the same drum for variable configurations
- Improves operational efficiency by reducing material preparation time and increasing operator safety
- Minimizes caking of hygroscopic salts
- Offers triple protection: against moisture inside and outside, and against contamination
- This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
- PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek® liner
- Desiccant bags are firmly integrated into the polyethylene liner
- Triple protection delivers two invaluable results:
- Dramatically reduced caking
- No contamination risk from the desiccant
Physical form
Lyophilized, spray dried, or crystallized material.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and Company
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Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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