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Merck

HT10132

Bouin′s solution

histological fixative

Synonym(s):

Bouin’s fluid

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47
MDL number:
MFCD00146169

Key Documents

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Product Name

Bouin′s solution, histological fixative

shelf life

Expiry date on the label

composition

acetic acid, 5%
formaldehyde, 9%
picric acid, 0.9%

color

yellow

application(s)

hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Bouin′s solution is an excellent fixative for preserving soft and delicate structures. It is used as a mordant in various trichrome procedures.
Additionally, it has been used for the following purposes-
  • staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
  • fixation of rat ovaries for the observing follicle cells using microscopy
  • fixation of gonads of crab for morphological and histological analysis
Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Biochem/physiol Actions

Bouin′s solution causes shrinkage of tissues when used as a preservative due to its picric acid component. It preserves morphological and nuclear information in tissues upon fixation.

General description

Bouin′s solution is a noncoagulant yellow stain fixative that consists of saturated picric acid, formaldehyde, and acetic acid.

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

50-00-0 + 67-56-1

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Certificates of Analysis (COA)

Lot/Batch Number
0000480539
0000480539
0000336639
0000336639
0000250408

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