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P3397

Phosphoglucomutase from rabbit muscle

Name: Phosphoglucomutase from rabbit muscle
Brand: SIGMA

ammonium sulfate suspension, ≥100 units/mg protein

Synonym(s):

PGM, α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase

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About This Item

CAS Number:

9001-81-4

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

5.4.2.2 (BRENDA, IUBMB)

MDL number:

MFCD00131886

Specific activity:

≥100 units/mg protein

Biological source:

rabbit muscle

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Properties

biological source

rabbit muscle

Quality Level

300

description

ammonium sulfate suspension, >=100 units/mg protein

form

ammonium sulfate suspension

specific activity

≥100 units/mg protein

storage condition

(Tightly closed)

technique(s)

activity assay: suitable

color

white to light yellow

foreign activity

lactic dehydrogenase ≤0.5%, phosphoglucose isomerase ≤0.01%, pyruvate kinase ≤0.05%

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Research area: Cell Signaling

Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.

Application

Phosphoglycomutase has been used:

to study glycogenesis and phosphoglycomutase deficiency in humans
in phosphoglucomutase assays to study metabolic regulation
for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells
in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3)
in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP⁺)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi)

Biochem/physiol Actions

Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P).

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄, pH 6.0 containing 0.01% EDTA

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

7783-20-2

CAS No.

rmu

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Certificates of Analysis (COA)

Lot/Batch Number

0000542424

0000534687

0000528329