Y1501
Yeast Synthetic Drop-out Medium Supplements
without uracil
Synonym(s):
Synthetic drop-out medium for yeast
Product Name
Yeast Synthetic Drop-out Medium Supplements, without uracil
form
powder
application(s)
food and beverages
Quality Level
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Application
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids are present at a concentration of 76 mg/L except for leucine, which is present at 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)
Amino acids: All standard amino acids are present at a concentration of 76 mg/L except for leucine, which is present at 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)
Preparation Note
1.92 g will supplement 1 liter of medium.
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Vinicius Henrique De Oliveira et al.
Ecotoxicology and environmental safety, 202, 110917-110917 (2020-08-18)
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Dhammika H M L P Navarathna et al.
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Hangrui Liu et al.
Biotechnology and bioengineering, 118(2), 647-658 (2020-10-07)
Yeast has been engineered for cost-effective organic acid production through metabolic engineering and synthetic biology techniques. However, cell growth assays in these processes were performed in bulk at the population level, thus obscuring the dynamics of rare single cells exhibiting
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