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Merck

NA13

Anti-Replication Protein A (Ab-1) Mouse mAb (RPA70-9)

liquid, clone RPA70-9, Calbiochem®

동의어(들):

Anti-RP-A

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
RPA70-9, monoclonal
Species reactivity:
human, yeast
Application:
Citations:
23
기술 서비스
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biological source

mouse

Quality Segment

antibody form

purified antibody

antibody product type

primary antibodies

clone

RPA70-9, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, yeast

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Frozen Sections (2.5 µg/mL)
Immunoblotting (1-5 µg/mL)
Immunofluorescence (2.5 µg/mL)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2.5 µg/mL, no pre-treatment required))

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... RPA1(6117)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with the NS1 myeloma cell line. Recognizes the ~70 kDa Replication Protein A.
Recognizes the ~70 kDa subunit of replication protein A in HeLa and u293 cells and colon carcinoma tissue.
This Anti-Replication Protein A (Ab-1) Mouse mAb (RPA70-9) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of Replication Protein A (Ab-1).

Immunogen

Epitope: Within the p70 subunit of replication protein A
Human
replication protein A purified from U293 cells

Application


Frozen Sections (2.5 g/ml)
Immunoblotting (1-5 g/ml)
Immunofluorescence (2.5 g/ml)
Immunoprecipitation (1 g/reaction)
Paraffin Sections (2.5 g/ml, no pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Positive Control
HeLa or U293 cells or colon carcinoma tissue

Other Notes

Din, S., et al. 1990. Genes Dev.4, 968.
Brill, S.J. and Stillman, B., 1989. Nature342, 92.
Stillman, B., 1989. Annu. Rev. Cell. Biol.5, 197.
Tsurimoto, T. and Stillman, B., 1989. EMBO J.8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA84, 1834.
Specific for the p70 subunit of replication protein A. This antibody can be used as a marker for proliferation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


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저장 등급

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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