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CELLMM2

FLAG® M Purification Kit

For Mammalian expression systems.

Synonym(s):

Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
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Quality Level

200

technique(s)

protein extraction: suitable

shipped in

wet ice

storage temp.

−20°C

Application

The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
  • ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
  • Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Packaging

Sufficient for 3-5 uses of 1-ml affinity purification column.

Other Notes

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • C2978
    CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
    SDS
  • SAE0194
    Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powder
    SDS
  • A2220
    ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution
    SDS
  • C2103
    Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.
    SDS

Storage Class

10 - Combustible liquids

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000524039
0000524039
0000240927
0000240927
0000176020

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