09-818
Anti-CARM1 Antibody
from rabbit
Synonym(s):
Protein arginine N-methyltransferase 4, coactivator-associated arginine methyltransferase 1, coactivator-associated arginine methyltransferase-1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Species reactivity:
rat, human
Citations:
18
Technique(s):
western blot: suitable
Uniprot accession no.:
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human
species reactivity (predicted by homology)
mouse
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CARM1(10498)
mouse ... Carm1(59035)
Analysis Note
Control
Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Application
Anti-CARM1 Antibody is a rabbit polyclonal antibody for detection of CARM1 also known as Protein arginine N-methyltransferase 4, coactivator-associated arginine methyltransferase 1 & has been validated in WB.
Western Blot Analysis:
A 1 μg/mL dilution of this lot detected CARM1 in HeLa nuclear extracts. (Figure A).
A 1 μg/mL dilution of this lot detected CARM1 in HeLa nuclear extracts. (Figure A).
Biochem/physiol Actions
Recognizes CARM1, Mr ~60 kDa.
General description
CARM1 (coactivator-associated arginine methyltransferase 1) is an arginine N-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arginine residues within proteins. CARM1 targets include methylation of histone H3 at Arg17 and p300 at Arg2142. CARM1 is recruited to promoters upon gene activation together with histone acetyltransferases from the p300 and p160 families. During monocyte inflammatory stimulation, CARM1 acts together with p300 as a coactivator for NF-kB. It is responsible for moving cells toward the inner cell mass in developing blastocysts. CARM1 also plays an important role in androgen receptors and may play a role in prostate cancer progression.
~60 kDa
Immunogen
Epitope: a.a. 595-608
KLH-conjugated, synthetic peptide containing the sequence (CSPMSIPTNTMHYGS), corresponding to a.a. 595-608 of human CARM1.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified by peptide affinity chromatography. Supplied at 1 mg/ml in 0.1 M Tris-Glycine (pH7.4) 15 mM NaCl, 0.05% sodium azide.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Maria-Elena Torres-Padilla et al.
Nature, 445(7124), 214-218 (2007-01-12)
It has been generally accepted that the mammalian embryo starts its development with all cells identical, and only when inside and outside cells form do differences between cells first emerge. However, recent findings show that cells in the mouse embryo
Heng Hong et al.
Cancer, 101(1), 83-89 (2004-06-29)
Coactivator-associated arginine methyltransferase 1 (CARM1) is a transcriptional coactivator of the androgen receptor (AR). It is involved in the regulation of the biologic functions of the AR. It remains to be determined whether CARM1 is involved in prostatic carcinogenesis. The
D Chen et al.
Science (New York, N.Y.), 284(5423), 2174-2177 (1999-06-26)
The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors
Adam Burton et al.
Cell reports, 5(3), 687-701 (2013-11-05)
Cell plasticity or potency is necessary for the formation of multiple cell types. The mechanisms underlying this plasticity are largely unknown. Preimplantation mouse embryos undergo drastic changes in cellular potency, starting with the totipotent zygote through to the formation of
Cynthia M Quintero et al.
Journal of molecular biology, 430(21), 4168-4182 (2018-08-29)
Activation of the retinoic acid (RA) signaling pathway is important for controlling embryonic stem cell differentiation and development. Modulation of this pathway occurs through the recruitment of different epigenetic regulators at the retinoic acid receptors (RARs) located at RA-responsive elements
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 09-818 | 04053252331923 |
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