M1302
M-MLV Reverse Transcriptase
Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis
Synonym(s):
Moloney Murine Leukemia Virus Reverse Transcriptase
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
Biological source:
Porcine intestinal mucosa
Recombinant:
expressed in E. coli
Concentration:
200 units/μL
biological source
Porcine intestinal mucosa
recombinant
expressed in E. coli
form
liquid
usage
sufficient for 200 reactions
feature
dNTPs included: no, hotstart: no
concentration
200 units/μL
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
Quality Level
Related Categories
Application
M-MLV Reverse Transcriptase has been used:
- for the preparation of cDNA libraries or for first strand cDNA synthesis
- for use in a 2-step RT-PCR assay
- in quantitative realtime-polymerase chain reaction (RT-qPCR)
- in reverse transcription
Features and Benefits
- Thermostable reverse transcriptase active at 37 °C.
- Can be used to generate first strand cDNA of up to 7 kb.
General description
Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
Other Notes
One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.
Packaging
Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.
Preparation Note
The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
Guzik-Kornacka A, et al.
Acta Neurobiologiae Experimentalis, 71(2), 193-207 (2011)
Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana
Dikaios I, et al.
Photosynthesis Research, 1-16 (2019)
Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
<BIG><BIG>Guzik KA, et al.</BIG></BIG>
Acta Neurobiologiae Experimentalis, 71, 193-207 (2011)
Murine leukemia virus reverse transcriptase: structural comparison with HIV-1 reverse transcriptase.
Marie L Coté et al.
Virus research, 134(1-2), 186-202 (2008-02-26)
Recent X-ray crystal structure determinations of Moloney murine leukemia virus reverse transcriptase (MoMLV RT) have allowed for more accurate structure/function comparisons to HIV-1 RT than were formerly possible. Previous biochemical studies of MoMLV RT in conjunction with knowledge of sequence
D S Howland et al.
Brain research. Molecular brain research, 11(3-4), 345-353 (1991-10-11)
The phosphoprotein synapsin I is expressed exclusively in neuronal cells. We are interested in elucidating the promoter sequences involved in cell type-specific expression of the synapsin I gene. The PC12 cell line expresses the 3.4 kb and 4.5 kb synapsin
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service