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RDD008

Trizma^® base

Name: Trizma<SUP>&#174;</SUP> base
Brand: SIGMA

≥99.9%, powder, anhydrous, Redi-Dri^™

Synonym(s):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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About This Item

Linear Formula:

NH₂C(CH₂OH)₃

CAS Number:

77-86-1

Molecular Weight:

121.14

NACRES:

NA.25

PubChem Substance ID:

329824219

UNSPSC Code:

12161700

EC Number:

201-064-4

MDL number:

MFCD00004679

Beilstein/REAXYS Number:

741883

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Properties

Product Name

Trizma^® base, anhydrous, free-flowing, Redi-Dri^™, ≥99.9%

grade

anhydrous

Quality Level

100

description

aminopeptidase substrate

product line

Redi-Dri^™

assay

≥99.9%

form

powder

quality

free-flowing

does not contain

Carbon Fiber

impurities

≤0.2% Water (Karl Fischer)

color

white

pH

10.5-12, 7-9

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (Water < 0.2%)

absorption

≤0.05 at 290 nm at 40%

λ

(A290 UV absorbance < 0.05), in 5 mM Tris; DNA (40% (w/w))

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:

To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
As a component of nuclei extraction buffer

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Extracellular protease digestion to evaluate membrane protein cell surface localization.

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Nature Protocols, 10(12), 2074-2074 (2015)

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