SBR00022
Blasticidin S
Ready Made Solution, 10 mg/mL (20 mM HEPES)
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About This Item
Empirical Formula (Hill Notation):
C17H27N8O5Cl
Molecular Weight:
458.90
UNSPSC Code:
12352106
NACRES:
NA.76
Quality Level
assay
≥98% (HPLC)
form
liquid, ready-to-use solution
concentration
10 mg/mL (20 mM HEPES)
color
colorless to faint yellow
antibiotic activity spectrum
fungi
mode of action
protein synthesis | interferes
storage temp.
−20°C
SMILES string
CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
InChI
1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H
InChI key
YQXYQOXRCNEATG-UHFFFAOYSA-N
General description
Blasticidin S, originally isolated from S. griseochromogenes, is a bacterial metabolite renowned for its antibacterial and fungicidal activities. This compound serves as a potent inhibitor of protein synthesis, exhibiting activity against various microorganisms, including bacteria (B. subtilis, S. lutea, E. coli, P. fluorescens, and M. tuberculosis), tumor cell lines, and nematodes.
In research, Blasticidin S has become a valuable tool, notably as a marker for strain manipulations. Recent applications involve the use of Blasticidin S as a selection agent for cells carrying plasmids conferring blasticidin resistance. The resistance is mediated by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus). These genes produce enzymes that catalyze the hydrolytic deamination of the cytosine moiety in blasticidin S, leading to the formation of a non-toxic deaminohydroxy derivative. This resistance mechanism is crucial in various studies involving genetic manipulations and selections in cell biology and biochemical research.
In research, Blasticidin S has become a valuable tool, notably as a marker for strain manipulations. Recent applications involve the use of Blasticidin S as a selection agent for cells carrying plasmids conferring blasticidin resistance. The resistance is mediated by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus). These genes produce enzymes that catalyze the hydrolytic deamination of the cytosine moiety in blasticidin S, leading to the formation of a non-toxic deaminohydroxy derivative. This resistance mechanism is crucial in various studies involving genetic manipulations and selections in cell biology and biochemical research.
Application
Blasticidin S has been:
- studies to have fungicidal properties and prevents rice blast disease.
- used as a selection agent for transformed cells that contain the resistance genes bls, bsr, or bsd. Blasticidin S has been used to select HEK293-T cells with TLR-2 constructs and HEK-D5 cells.
- used to study protein synthesis at the level of peptide bond formation.
Biochem/physiol Actions
Mode of Action: It inhibits protein synthesis in bacteria and eukaryotes. Blasticidin S inhibits hydrolysis of peptidyl-tRNA induced by release factors. It enhances the binding of tRNA to the large subunit of ribosomes and to an extent inhibits peptide bond formation.
Antimicrobial Spectrum: Active against mycobacteria, several Gram-positive and Gram-negative bacteria
Antimicrobial Spectrum: Active against mycobacteria, several Gram-positive and Gram-negative bacteria
Features and Benefits
- High-quality antibiotic suitable for multiple research applications
- Ideal for Cell Biology and Biochemical research
Other Notes
For additional information on our range of Biochemicals, please complete this form.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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Martha Triantafilou et al.
PloS one, 8(4), e61199-e61199 (2013-04-18)
Ureaplasma species are the most frequently isolated microorganisms inside the amniotic cavity and have been associated with spontaneous abortion, chorioamnionitis, premature rupture of the membranes (PROM), preterm labour (PL) pneumonia in neonates and bronchopulmonary dysplasia in neonates. The mechanisms by
Egor Svidritskiy et al.
Journal of molecular biology, 430(5), 591-593 (2018-01-26)
Understanding the mechanisms of inhibitors of translation termination may inform development of new antibacterials and therapeutics for premature termination diseases. We report the crystal structure of the potent termination inhibitor blasticidin S bound to the ribosomal 70S•release factor 1 (RF1)
Suneesh Kumar Pachathundikandi et al.
PloS one, 6(5), e19614-e19614 (2011-05-17)
Helicobacter pylori is the causative agent for developing gastritis, gastric ulcer, and even gastric cancer. Virulent strains carry the cag pathogenicity island (cagPAI) encoding a type-IV secretion system (T4SS) for injecting the CagA protein. However, mechanisms of sensing this pathogen