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Merck

TOX8

In Vitro Toxicology Assay Kit, Resazurin based

Synonym(s):

Fluorescent resazurin toxicology assay

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About This Item

UNSPSC Code:
12352207
EC Number:
263-718-5
NACRES:
NA.84
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form

liquid

Quality Level

usage

1 kit sufficient for 2,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

protein quantification: suitable

application(s)

cell analysis
detection

detection method

colorimetric, fluorometric

storage temp.

2-8°C

General description

In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible

Application

In Vitro Toxicology Assay Kit, Resazurin based has been used:
  • in cell viability assay of human primary fibroblasts
  • to determine the cell proliferation rate in murine pre-osteoblasts
  • to determine the metabolic activity of microfluidic and monolayer cultures

Biochem/physiol Actions

Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).


Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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U J Strotmann et al.
Ecotoxicology and environmental safety, 25(1), 79-89 (1993-02-01)
The dehydrogenase assay with resazurin as an oxidoreduction dye was used for monitoring the performance of six laboratory-scale sewage treatment plants. The dehydrogenase activity measured proved to be a reliable monitoring parameter, although it did not always positively correlate with
José Manuel Quesada-Gómez et al.
The Journal of steroid biochemistry and molecular biology, 148, 214-218 (2014-12-03)
The nitrogen-containing or nitrogenous bisphosphonates (N-BF) are currently the main class of drugs used for the treatment of diseases characterized by an increased bone resorption. Preliminary data suggest that N-BF have also direct or indirect anti-tumoral effects, and recent evidence
Comparison of several microbiological toxicity screening tests.
Dutka, B.J., et al.
Water Research, 17, 1363-1367 (1983)