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D7008

Dihydroethidium

Name: Dihydroethidium
Brand: SIAL

≥95% purity, powder

Synonym(s):

2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₂₁N₃

CAS Number:

104821-25-2

Molecular Weight:

315.41

NACRES:

NA.47

PubChem Substance ID:

24894069

UNSPSC Code:

12171500

MDL number:

MFCD00077335

Beilstein/REAXYS Number:

5107482

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Properties

Product Name

Dihydroethidium, ≥95%

Quality Segment

100

assay

≥95%

form

powder

technique(s)

titration: suitable

color

pink to very dark red

solubility

chloroform: 20 mg/mL

fluorescence

λ_ex 370 nm; λ_em 420 nm (for cytoplasm of living cells), λ_ex 535 nm; λ_em 610 nm (for chromatin of living cells)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

Description

Application

Redox indicator. Blue fluorescence until oxidized to ethidium.

Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).

Packaging

Packaged under Argon.

Other Notes

B-ring reduced analog of ethidium

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c