12306C
Fetal Bovine Serum
non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture
Synonym(s):
HI FBS, HI FCS, HI Fetal Bovine Serum, HI Fetal Calf Sera, Heat Inactivated Fetal Calf Sera, FBS, FCS, sera, serum
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About This Item
Biological source:
bovine fetus
Origin:
non-USA origin
Sterility:
sterile-filtered
biological source
bovine fetus
sterility
sterile-filtered
product line
FBS Premier
quality
from USDA approved countries
composition
Hemoglobin, ≤25 mg/dL
origin
non-USA origin
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
storage temp.
−20°C
Quality Level
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Application
FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used to block the human induced pluripotent stem cells (hiPSCs) derived hepatocyte-like cells (HLCs) cells in phosphate buffered saline during immunofluorescence staining.
USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.
Biochem/physiol Actions
Fetal Bovine Serum (FBS) is used as a supplement to cell culture media. It provides many non-defined growth promoting and survival enhancing factors to cells in culture.
Analysis Note
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Generation of functional hepatocyte-like cells from human pluripotent stem cells in a scalable suspension culture
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Stem Cells and Development, 22(20), 2693-2705 (2013)
Differentiation and transplantation of human induced pluripotent stem cell-derived hepatocyte-like cells
Asgari S, et al.
Stem Cell Reviews and Reports, 9(4), 493-504 (2013)
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RNA modification in the form of N6-methyladenosine (m6A) regulates nearly all the post-transcriptional processes. The asymmetric m6A deposition suggests that regional methylation may have distinct functional consequences. However, current RNA biology tools do not distinguish the contribution of individual m6A
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ALTEX, 20(4), 275-281 (2003-12-13)
Serum is commonly used as a supplement to cell culture media. It provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors. The
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