B3804
BCIP®/NBT-Blue
alkaline phosphatase substrate, chromogenic, liquid
Synonym(s):
5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Product Name
BCIP®/NBT-Blue Liquid Substrate System for Membranes, alkaline phosphatase substrate
Quality Level
form
liquid
storage temp.
2-8°C
General description
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.
Application
BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.
Other Notes
Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Stable at least one year at 2-8 °C. This substrate is light-sensitive.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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