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B6024

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

Name: 5-Bromo-4-chloro-3-indolyl &#946;-<SC>D</SC>-galactopyranoside
Brand: SIGMA

tablet, suitable for identification of lac+ bacterial colonies

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₅BrClNO₆

CAS Number:

7240-90-6

Molecular Weight:

408.63

NACRES:

NA.83

PubChem Substance ID:

57653950

UNSPSC Code:

12352204

EC Number:

230-640-8

MDL number:

MFCD00005666

Beilstein/REAXYS Number:

1402009

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Properties

Product Name

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, tablet

form

tablet

Quality Level

200

suitability

suitable for identification of lac⁺ bacterial colonies

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

Description

General description

5-Bromo-4-chloro-3-indolyl β -D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for β-galactosidase.

Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used in the preparation of X-Gal stock solution to add it in LB agar plates for blue-white screening of transformants.

Biochem/physiol Actions

X-gal is routinely used to detect transgene activity in situ.

Preparation Note

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.

Contains 5 mg substrate per tablet

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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