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About This Item
usage
sufficient for 70 purifications
Quality Level
technique(s)
DNA purification: suitable
input
DNA (single or double stranded PCR ampliflied)
test parameters
sample volume: 10 μg, sample volume: 100 μL
storage temp.
15-25°C
General description
DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Application
Biochem/physiol Actions
Features and Benefits
- Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Removes over 99% of primers and other components
- Eliminates the need to remove mineral oil by organic extraction
- 40% more purification preps supplied than market leader
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Purifies up to 100 μL or 10 μg of PCR amplified DNA in 8 minutes
- Removes over 99% of primers and other components
Other Notes
Legal Information
Kit Components Also Available Separately
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 2
Storage Class
10 - Combustible liquids
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Protocols
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法,
WTA2(一种全转录组扩增(WTA)方法)能够在区区4小时内,代表性地扩增出纳克级的总RNA而没有3'偏差。
Articles
Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing.
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Related Content
Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
从细胞、组织、血液、病毒等样品类型中提取和纯化基因组DNA、质粒DNA和总RNA的常用技术及其下游应用的概述。

