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Merck

MABE648

Anti-Histone H3 pan Antibody, clone RM188

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
RM188, monoclonal
Species reactivity:
human, rabbit
Application:
WB, multiplexing
Citations:
-
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antibody form

purified antibody

Quality Segment

clone

RM188, monoclonal

purified by

using Protein A

species reactivity

human, rabbit

concentration

(Please refer to lot specific datasheet.)

technique(s)

multiplexing: suitable, western blot: suitable

isotype

IgG

UniProt accession no.

General description

A peptide corresponding to the C-terminus of human Histone H3.
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Application

Epitope: C-terminus

Biochem/physiol Actions

This antibody reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins.

Physical form

Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Evaluated by Western Blotting in recombinant Histone H3.3 protein, recombinant Histone H3.1 protein, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates.

Western Blotting Analysis (WB): 0.25 μg/mL of this antibody detected Histone H3 in Recombinant Histone H3.3 protein, recombinant Histone H3.1 protein, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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