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SCM313

3dGRO® Human Liver Organoid Progenitor Expansion Media

Synonym(s):

Hepatic PDOs, Hepatocyte Organoids, Human Hepatic Organoids, Human Liver PDOs

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.51
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biological source

human

Quality Segment

100

packaging

vial of 50 mL

manufacturer/tradename

Millipore

technique(s)

cell culture | stem cell: suitable

shipped in

dry ice

storage temp.

−20°C

General description

3dGRO® Human Liver Organoid Progenitor Expansion Media is a complete ready-to-use serum-free medium for the expansion and long-term culture of human iPSC derived liver organoids. The medium has been validated for use with 3dGRO® Human Liver Organoid Progenitors (Cat. No. SCC572). Liver Organoid Progenitors cultured in SCM313 express key liver progenitor markers HNF4a, SOX17, FOXA2, SOX9, cytochrome P450 (Cyp) enzyme activities and proper apical and basal-lateral polarization marked by E-cadherin and ZO1 expression.

Application

  • Appearance (Color): Clear/No Particlulates (Red Liquid)
  • Osmolality: 350-375 mOsm
  • pH: 7.0 – 7.4
  • Sterility Tested: No Growth/Pass
  • Endotoxin: 2 EU/mL
  • Mycoplasma: Negative
  • Functional Assay: Thaw and culture of human iPSC derived liver organoids for 2 passages.

Features and Benefits

Complete serum-free organoid media for the expansion and long-term 3D culture of human iPSC derived liver organoids.

Preparation Note

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

Other Notes

3dGRO® is a trademark of Merck KGaA, Darmstadt, Germany.

Legal Information

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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