SLM-021
EmbryoMax® DMEM (1X), liquid, With 4,500mg/L Glucose, without L-Glutamine and Sodium Pyruvate
The EmbryoMax DMEM with 4,500mg/L Glucose, without L-Glutamine & Sodium Pyruvate is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
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About This Item
form
liquid
manufacturer/tradename
Specialty Media, EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type neural stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type cardiac stem cell(s)
sample type: human embryonic stem cell(s)
Preparation Note
Legal Information
Disclaimer
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Protocols
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
Related Content
The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.
In order to create a quantitatively defined diffusion gradient that is stable enough for long-term, live cell imaging over the course of days, we developed a microfluidic gradient plate. This plate, designed for use on the CellASIC™ ONIX Microfluidic Platform, enables precision-controlled chemoattractant diffusion across perfusion barriers to create a spatial gradient in the culture area. On the other end of each culture area, there are flow outlets. Together, the perfusion inlets and outlets form a continuous flow “infinite source/sink” that maintains a stable concentration gradient profile for days. The flexible format of the plate enables changes in gradient directionality, turning gradients on and off, and toggling between gradient and single solution exposure.