E9030
Endoglycoceramidase II from Rhodococcus sp.
aqueous solution
Synonym(s):
EGCase, ceramide glycanase, glycosyl-N-acetyl-sphingosine 1,1-β-D-glucanohydrolase, oligoglycosylglucosyl(1↔1)ceramide glycohydrolase, oligoglycosylglucosylceramide glycohydrolase
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About This Item
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
conjugate
(Lipid-linked)
form
solution
mol wt
58.9 kDa
shipped in
dry ice
storage temp.
−20°C
Application
Endoglycoceramidase II from Rhodococcus sp. has been used in a study to assess the differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography and mass spectrometry. Endoglycoceramidase II from Rhodococcus sp. has also been used in a study to investigate structural and mechanistic analyses of endo-glycoceramidase II.
Physical form
Solution in 20 mM sodium acetate buffer, pH 6.0, containing 0.2% BSA and 0.1% Lubrol PX.
Other Notes
One unit will hydrolyze 1 μmol of asialo-GM1 per min at 37 °C at pH 5.0.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Related Content
Nahid Azzouz et al.
International journal for parasitology, 32(6), 677-684 (2002-06-14)
Glycolipids are important components of cellular membranes involved in various biological functions. In this report, we describe the identification of the de novo synthesis of glycosphingolipids by Toxoplasma gondii tachyzoites. Parasite-specific glycolipids were identified by metabolic labelling of parasites with
Matthew E C Caines et al.
The Journal of biological chemistry, 282(19), 14300-14308 (2007-03-03)
endo-Glycoceramidase, a membrane-associated family 5 glycosidase, deviates from the typical polysaccharide substrate specificity of other soluble members of the family, preferentially hydrolyzing glycosidic linkages between the oligosaccharide and ceramide moieties of gangliosides. Here we report the first x-ray crystal structures
A E Manzi
Current protocols in molecular biology, Chapter 17, Unit17-Unit17 (2008-02-12)
Selective cleavage of the oligosaccharide moiety of glycolipids for further structural analysis can be achieved by means of endoglycoceramidase (EGCase), an enzyme specific for the linkages between oligosaccharide and ceramide residues in glycolipids. The method described here can be used