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F0291

Fibroblast Growth Factor-Basic, human

Name: Fibroblast Growth Factor-Basic, human
Brand: SIGMA

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

Fibroblast Growth Factor-Basic human, FGF2, bFGF, hBFGF

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About This Item

CAS Number:

106096-93-9

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00081382

Biological source:

human

Recombinant:

expressed in E. coli

Assay:

≥97% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

16.0 kDa

Impurities:

≤1.00 EU/μg

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Properties

Product Name

hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

biological source

human

Quality Level

200

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤-0.6 ng/mL

mol wt

16.0 kDa

packaging

pkg of 4X25 μg, pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg

color

white to faint yellow cast

solubility

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt accession no.

P09038

storage temp.

−20°C

Gene Information

human ... FGF2 (2247)

Description

General description

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.

Application

Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).

Physical form

Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.

Analysis Note

The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Growth Factors in Stem Cell Biology

Role of growth factors in stem cell differentiation and various growth factors for your research at sigmaaldrich.com

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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Activin A maintains self-renewal and regulates fibroblast growth factor, Wnt, and bone morphogenic protein pathways in human embryonic stem cells.

Lei Xiao et al.

Stem cells (Dayton, Ohio), 24(6), 1476-1486 (2006-02-04)

Human embryonic stem cells (hESCs) self-renew indefinitely while maintaining pluripotency. The molecular mechanism underlying hESCs self-renewal and pluripotency is poorly understood. To identify the signaling pathway molecules that maintain the proliferation of hESCs, we performed a microarray analysis comparing an

Feeder-free growth of undifferentiated human embryonic stem cells.

C Xu et al.

Nature biotechnology, 19(10), 971-974 (2001-10-03)

Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least

Adhesion profile and differentiation capacity of human adipose tissue derived mesenchymal stem cells grown on metal ion (Zn, Ag and Cu) doped hydroxyapatite nano-coated surfaces.

R Beklem Bostancioglu et al.

Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)

Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human

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