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L1882

Lectin from Triticum vulgaris (wheat)

Name: Lectin from <I>Triticum vulgaris </I>(wheat)
Brand: SIGMA

agarose conjugate, saline suspension

Synonym(s):

WGA, Wheat germ agglutinin

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

Form:

saline suspension

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Properties

conjugate

agarose conjugate

Quality Level

200

form

saline suspension

extent of labeling

5-10 mg per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

capacity

≥0.1 mg/mL binding capacity (ovomucoid)

storage temp.

2-8°C

Description

General description

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.

Physical form

Suspension in 1.0 M NaCl containing 15 mM sodium azide

Preparation Note

Conjugates are prepared from affinity purified lectin.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves

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Protocols

用于亲和层析的色谱柱填充和制备实验方案

该页面展示了如何使用Cytiva的Tricorn™或XK色谱柱进行亲和层析色谱柱填充和制备。

线性流速（cm/小时）与体积流速（mL/min)之间的换算

本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

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Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.