P5431
Pectolyase from Aspergillus japonicus
aseptically filled, lyophilized powder, ≥2 units/mg solid
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About This Item
sterility
aseptically filled
form
lyophilized powder
specific activity
≥2 units/mg solid
storage temp.
2-8°C
Application
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Physical form
Lyophilized powder containing lactose
Preparation Note
Prepared from P 3026
Other Notes
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.
Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Ross G Atkinson et al.
BMC plant biology, 12, 129-129 (2012-08-04)
While there is now a significant body of research correlating apple (Malus x domestica) fruit softening with the cell wall hydrolase ENDO-POLYGALACTURONASE1 (PG1), there is currently little knowledge of its physiological effects in planta. This study examined the effect of
Mahejibin Khan et al.
Annual review of food science and technology, 4, 21-34 (2012-11-30)
The understanding that enzymatic degradation of fruit pectin can clarify juices and improve juice yields resulted in the search for microbial pectinases and application in vegetable- and fruit-processing industries. Identified enzymes were classified on the basis of their catalytic activity
J D P Bezerra et al.
World journal of microbiology & biotechnology, 28(5), 1989-1995 (2012-07-19)
Opuntia ficus-indica Mill. (forage cactus) is farmed with relative success in the semi-arid region of the Brazilian northeast for commercial purposes, particularly as forage and food. Endophytic microorganisms are those that can be isolated inside plant tissues and can be