Quality Level
technique(s)
whole genome amplification: suitable
dilution
(WTA)
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
General description
用于全转录组扩增(WTA)的SeqPlex™RNA扩增试剂盒(SEQXE)专用于对少量或降解/高度片段化的RNA（如来自福尔马林固定石蜡包埋（FFPE）组织样本的RNA ）进行下一代测序(NGS)。通过SeqPlex试剂盒可对这类RNA以及其它用于NGS的少量/高度片段化RDNA样品进行预扩增。它还有助于扩增从组织、培养细胞、福尔马林固定样品或血清中分离的非 polyA 尾 RNA，同时保持未扩增样品中发现的差异表达模式。
Application
SeqPlex™ RNA扩增试剂盒已用于扩增poly（A）筛选的RNA。
Features and Benefits
- 少量总 RNA 随机引物技术可扩增来自所有来源（包括 FFPE 和 RIP）的片段化或完整 RNA。
- 半简并文库引物设计，可实现更完整的转录组覆盖和高效引发。
- 测序前无需进行 DNA的片段化。
- 在 8 小时或更短的时间内扩增 ds-cDNA。
- 兼容Pacific Bioscience以外的其它所有下一代测序平台。
Other Notes
SEQR-500RXN 按需生产。请联系我们获取更多信息。
Legal Information
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
实验方案
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
相关内容
Instructions
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Meritxell Jodar et al.
Human reproduction update, 19(6), 604-624 (2013-07-17)
BACKGROUND Spermatozoa are highly differentiated, transcriptionally inert cells characterized by a compact nucleus with minimal cytoplasm. Nevertheless they contain a suite of unique RNAs that are delivered to oocyte upon fertilization. They are likely integrated as part of many different