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Merck

70922

BugBuster® HT蛋白提取试剂

别名:

细胞裂解试剂, 蛋白质提取缓冲液

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关于此项目

NACRES:
NA.77
UNSPSC Code:
41106511
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form

liquid

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

2-8°C

General description

BugBuster HT蛋白质提取试剂将BugBuster蛋白质提取试剂和Benzonase®核酸酶结合在一个方便的试剂中。BugBuster HT消除了传统细胞裂解程序引起的常见生物加工问题。从大肠杆菌中轻轻提取可溶性蛋白质,而无需暴露于热或氧化损伤,并且可以通过一步核酸消化消除粘度。所得的蛋白质提取物可以通过常规纯化技术容易地分级分离。BugBuster HT非常适合用于高通量蛋白质纯化。

Application

BugBuster® HT蛋白质提取试剂已用于:
  • 在离心进行蛋白质表达和免疫印迹后裂解大肠杆菌细胞颗粒
  • 从细菌细胞颗粒中提取蛋白质
  • 裂解大肠杆菌细胞颗粒,用于表达和纯化dMIC60(线粒体内膜蛋白)蛋白

Features and Benefits

  • 快速蛋白质提取和核酸降解
  • 非常适合处理不同体积的各种样品
  • 添加r溶菌酶溶液可确保提高提取率

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

毒性:标准处理(A)


存储类别

10 - Combustible liquids

wgk

WGK 2



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商品

The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

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E. coli transformation with poly-histidine tagged constructs represents a common vehicle for protein production. Initial screening of small-scale cultures is routinely performed to identify clones producing the highest amounts of protein with the desired form and function. As the demand for greater throughput at this level has increased, so has the need for process simplification and greater reproducibility. To meet this need, we have developed a condensed workflow that can be performed in a single device.

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全球贸易项目编号

货号GTIN
70922-3CN04055977272758
70922-4CN04055977272765
70922-5CN04055977272192