sterility
sterile-filtered
Quality Level
form
liquid
shelf life
Expiry date on the label.
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
density
1.077
application(s)
hematology
histology
storage temp.
2-8°C
General description
Histopaque-1077 是一种无菌、经过内毒素测试的即用型培养基。Histopaque组合液包括聚蔗糖和泛影酸钠，密度经调节后为1.077g/mL。这种即用型试剂有助于从小体积的全血或骨髓中进行活性淋巴细胞和其他单个核细胞的快速恢复。
Application
Histopaque-1077用于：
- 人淋巴细胞抗原(HLA)分型
- T、B和‘裸’淋巴细胞计数前的起始分离。
- 制备用于细胞培养和细胞毒性试验的纯淋巴细胞悬液。
- 分离和纯化外周血单个核细胞(PBMC)和骨髓单个核细胞(BMMC)
- 分离人类淋巴细胞
- 分离全血中乳癌循环肿瘤细胞(CTC)
- Histopaque-1077适合与Histopaque-1119一起使用，因为其有助于从少量全血中快速恢复活性单个核细胞和粒细胞
Biochem/physiol Actions
将抗凝血铺在Histopaque-1077液面上。离心时，红细胞被聚蔗糖聚集并迅速沉淀。粒细胞变得略微高渗，它们的沉降速率增加，在ACCUSIN管底部形成沉淀。淋巴细胞和其他单个核细胞留在血浆/Histopaque-1077分界面。
Features and Benefits
Histopaque®-1077 有许多优势，比如：
- 可使活细胞极佳恢复
- 允许选择分离不同种类的血细胞
- 防止细胞变形
- 保持细胞活力
- 最小化外来细胞干扰
- 各批次性能可重复
- 经过过滤杀菌和内毒素检测
- 在 2-8°C 避光保护条件下可稳定保存至少2年
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
实验方案
Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。
本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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