sterility
sterile-filtered
Quality Level
form
liquid
shelf life
Expiry date on the label.
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
density
1.119
application(s)
hematology
histology
storage temp.
2-8°C
General description
Histopaque®-1119 是一种无菌、经过内毒素测试的即用型培养基。该溶液由聚蔗糖和泛影酸钠组成，调配的密度为1.119 g/mL，pH为8.8–9.0。
Application
Histopaque®-1119适合与Histopaque-1077一起使用，因为它有助于从少量全血或骨髓中快速恢复活性单个核细胞和粒细胞。
Biochem/physiol Actions
该过程涉及在 Histopaque-1119 上使等体积的 Histopaque-1077 分层，形成双梯度。将抗凝全血小心地铺在上层 Histopaque-1077 培养基上。离心后，红细胞被聚蔗糖聚集并因此迅速沉淀。粒细胞位于下部Histopaque-1077/1119 界面； 而在上部血浆/Histopaque-1077 界面处有淋巴细胞和其它单核细胞。在上部界面中发现的大多数外部血小板在洗涤步骤中通过低速离心去除。红细胞污染可以忽略不计。
Features and Benefits
Histopaque®-1119 有许多优势，比如：
- 可使活细胞极佳恢复
- 允许选择分离不同种类的血细胞
- 防止细胞变形
- 保持细胞活力
- 最小化外来细胞干扰
- 各批次性能可重复
- 经过过滤杀菌和内毒素检测
- 在 2-8°C 避光保护条件下可稳定保存至少2年
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
10 - Combustible liquids
实验方案
Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。
本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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