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Merck

17783

钙黄绿素-AM

suitable for fluorescence, BioReagent, ≥90% (HPLC)

别名:

钙黄绿素乙酰氧基甲酯, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester

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关于此项目

经验公式(希尔记法):
C46H46N2O23
化学文摘社编号:
分子量:
994.86
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
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product line

BioReagent

Quality Level

assay

≥90% (HPLC)

form

solid

mol wt

994.86 g/mol

fluorescence

λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3

InChI key

XKFSBWQWNMZWFA-UHFFFAOYSA-N

General description

脂质体含有一种自淬灭荧光染料,称为钙黄绿素。它是一种荧光染料,激发和发射波长分别为495/515 nm。它在浓度高于70mM时发生自淬灭,通常用作脂质囊泡渗漏的指示剂。当捕获的钙黄绿素从脂质体区室泄漏时,可根据荧光增加了计算泄漏速率。它被用作络合物指示剂,用来滴定EDTA螯合的钙离子,并且用于通过荧光检测法测定钙离子浓度。

Application

钙黄绿素-AM已被用于流式细胞术。
钙黄绿素的非荧光细胞渗透衍生物,水解后发出荧光。 用作多药耐药蛋白MRP的中性底物;用于肿瘤研究。


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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分析证书(COA)

Lot/Batch Number

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