biological source
bacterial (LYSOBACTER SP. BACTERIA M497-1)
Quality Level
form
lyophilized powder
specific activity
≥1,000 units/mg solid
mol wt
27 kDa
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
Application
消化肽酶可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。Sigma 的消化肽酶可用于皮肤和软组织细菌感染的分离和分子鉴定。
Biochem/physiol Actions
消化肽酶是赖氨酰内肽酶,其分子量约 27kDa。它在 pH 8.5-9之间效果最佳。可以使用大约 500-1,500 μn/ml 的无色肽酶裂解细胞,细胞密度 OD600 =0.6,37℃,超过 2 小时。
消化肽酶是赖氨酰内肽酶,分子量约 27kDa。它可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。
活性最佳的 pH 值:pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞,细胞密度 OD600=0.6,37℃,超过 2 小时 。
活性最佳的 pH 值:pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞,细胞密度 OD600=0.6,37℃,超过 2 小时 。
Physical form
粗粉
Other Notes
酶用缓冲液悬浮后,在pH 8.0、37 °C下,1单位每分钟可释放0.4 μg溶壁微球菌(浊度法检测,600nm)。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
实验方案
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
相关内容
查找细胞裂解和蛋白质提取试剂,如细胞裂解缓冲液、蛋白质提取试剂盒、提取酶和其他用于分离和纯化蛋白质的裂解增强剂。
Find cell lysis and protein extraction reagents like cell lysis buffers, protein extraction kits, extraction enzymes, and other lysis enhancers for isolating and purifying proteins.
Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
Shah, et al.
PLoS ONE, 18, e0284424-e0284424 (2023)
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Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
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