产品名称
DMEM/营养混合物 F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO3: no
phenol red: no
glucose: 3.15 g/L
shipped in
ambient
storage temp.
2-8°C
General description
提供的最基本的制剂。该制剂由希望从 (1;1) DME/Ham F-12 的必需组分开始,并具有为自身应用优化制剂的灵活性的研究者使用。该基础制剂特别适用于标记氨基酸的细胞蛋白代谢研究。
Preparation Note
补充 0.1545 g/L 氯化钙,0.365 g/L L -谷氨酰胺,0.05905 g/L L -亮氨酸,0.09125 g/L L -赖氨酸,0.0612 g/L 氯化镁,0.04884 g/L 硫酸镁,0.0172 g/L L -蛋氨酸,1.2 g/L 碳酸氢钠。
配制成每升培养基含 14.8 克粉末。
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存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性,应注意防潮。
商品
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
钙是一种离子稳定的二价阳离子,在细胞培养基中具有有益和有毒的双重属性,可作为一系列细胞培养基的组成部分。
Vincent J Munster et al.
Nature, 585(7824), 268-272 (2020-05-13)
An outbreak of coronavirus disease 2019 (COVID-19), which is caused by a novel coronavirus (named SARS-CoV-2) and has a case fatality rate of approximately 2%, started in Wuhan (China) in December 20191,2. Following an unprecedented global spread3, the World Health Organization
Yuuma Furutani et al.
Cell biochemistry and function, 29(7), 577-581 (2011-08-23)
Magnesium (Mg) deficiency induces the production of free radicals, increases cytosolic ionized calcium concentration, and modulates the function of skeletal muscle in rats. The present study examined the effects of Mg deficiency on the gene expression of molecules related to
Rui Yan et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 103, 1490-1497 (2018-06-06)
Neonatal brain hypoxia is a disease that affects the nervous system in children. Salidroside is a compound that has an anti-hypoxic effect, but the mechanism of salidroside in neonatal cerebral hypoxia is unclear. Hence, we investigated the regulatory effect and