I3769
Insulin-like Growth Factor-I human
≥95% (HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
别名:
IGF, IGF-I, somatomedin c
登录并加购，请在购物车里查看协议价、货期和发货地。
选择规格
变更视图
关于此项目
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥95% (HPLC)
Form:
lyophilized powder
Mol wt:
~7.6 kDa
Impurities:
≤1.00 EU/μg
产品名称
Insulin-like Growth Factor-I human, IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in E. coli
assay
≥95% (HPLC)
form
lyophilized powder
potency
0.1-10 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
~7.6 kDa
packaging
pkg of 50 and 100 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IGF1(3479), Igf1(16000)
Application
Insulin-like Growth Factor-I human has been used:
- in modified Dulbecco minimal essential medium for in vitro culture of testicular cells
- in culture media, to study its effect on steroidogenesis during testicular development
- in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation
Biochem/physiol Actions
Insulin-like growth factor I (IGF-I) plays a critical role in cellular energy metabolism and growth and development, especially prenatal growth.
IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.
IGF-I, also known as somatomedin C, mediates the growth-promoting activity of GH (growth hormone). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis. IGF-I exerts its actions through the IGF-I receptor.
Physical form
Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid.
Preparation Note
Reconstitute in water
Analysis Note
The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED50 corresponds to a specific activity of at least 1 x 105 units/mg.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)