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KCQS01

KiCqStart® SYBR® Green qPCR ReadyMix预混液™

Low ROX™, for ABI and Stratagene instruments

别名:

qPCR master mix, sybr green qPCR

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关于此项目

NACRES:
NA.55
UNSPSC Code:
41106300
技术服务
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form

liquid

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

feature

dNTPs included, hotstart

storage condition

protect from light

technique(s)

RT-qPCR: suitable, qPCR: suitable

color

colorless

input

purified DNA
crude DNA

compatibility

for use with ABI 7500 Fast, for use with ABI 7500, for use with ABI ViiA 7, for use with Agilent AriaMx, for use with Douglas Scientific IntelliQube, for use with Qiagen Rotor-Gene Q, for use with QuantStudio™, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000

detection method

SYBR® Green

shipped in

dry ice

storage temp.

−20°C

General description

KiCqStart SYBR Green qPCR ReadyMix是2倍浓缩的即用型预混液，含有除引物和模板之外的实时定量PCR(qPCR)所需的全部组分。这种专有缓冲液、稳定剂和Taq DNA热启动聚合酶的独特组合使用快速或常规的SYBR Green qPCR循环方案，具有最高的PCR效率、灵敏度、特异性和稳定可靠的荧光信号。

高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要，因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前，处于抗体介导的失活状态。

Application

KiCqStart® SYBR® Green qPCR ReadyMix™ has been used:
  • for the amplification and quantification of DNA in real-time PCR (qPCR) assay
  • in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR)
  • in the amplification of complementary DNA (cDNA) by real-time PCR (qPCR) assay
PCR扩增：
  • 基因表达
  • DNA定量
  • 染色质免疫沉淀(CHiP)

Features and Benefits

  • 只需33分钟即可获得分析结果
  • 高效、灵敏的实时PCR结果
  • 几乎不需要多少优化，甚至无需优化

Analysis Note

Kit components are free of contaminating DNase and RNase. KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (r2 > 0.995) and a PCR efficiency > 90%.

Other Notes

储存条件：
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存，可稳定存放1年。为方便使用，也可在+2至+8°C下储存长达6周。解冻后，充分混匀后再使用。不建议反复冻融产品。不过，冻融20次或+20°C存放2个月后，产品没有出现任何性能损失。
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Legal Information

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
QuantStudio is a trademark of IROA Technologies LLC
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

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存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
66283455
66218496
Q66218496
66261281
66206930

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实验方案

KiCqStart™通用SYBR® Green qPCR实验方案

使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。

使用温度梯度的引物优化试验方案

用于测定优化的梯度PCR通过在一系列退火温度下测试含有固定引物浓度的相同反应物来确定引物的最佳退火温度（Ta）。

qPCR效率测定实验方案

QPCR 条件的优化对于制定稳健的检测方案非常重要。两种主要优化方法是优化引物浓度和/或退火温度。

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商品

使用Imprint® RNA免疫沉淀试剂盒Ago RIP分离microRNA及其靶标

Sigma Imprint RNA 免疫沉淀试剂盒用于从 HeLa 细胞中共纯化人 argonaute 2 (Ago2) 相关 RNA。MicroRNA通过Mysticq 试剂进行逆转录和定量。

PCR 实验优化和验证

PCR测定指南可指导您全程了解引物验证和其他测定优化因素，确保实现理想的DNA / RNA定量高灵敏度和特异性。

聚合酶链反应：

聚合酶链反应是分子生物学应用最普遍的技术之一。PCR过程主要由三步组成：变性、退火和延伸

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