M8823
抗-FLAG® M2磁珠
affinity isolated antibody
别名:
单克隆抗-FLAG® M2 小鼠抗, FLAG®树脂，用于高通量, FLAG®磁性亲和树脂, Flag®亲和树脂, 抗 ddddk, 抗 dykddddk
选择尺寸
关于此项目
conjugate
magnetic beads
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
suspension
shelf life
2 yr at -20 °C
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
bead size
20-75 μm
matrix
superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
isotype
IgG1
capacity
≥0.6 mg/mL binding capacity
shipped in
wet ice
storage temp.
−20°C
General description
Application
Features and Benefits
- 极为快速的分离
- 显著被加速的操作，例如重复的洗涤
-以板式进行多个样本的处理
该可导致：
- 更为快速的实验
- 更好的可重复性
- 目标蛋白更为准确的定量
Physical form
Other Notes
Legal Information
Disclaimer
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flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
商品
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
FLAG®表达系统是一种表达、纯化和检测重组融合蛋白的成熟方法。作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。M2抗体可结合哺乳动物、细菌和植物提取物融合蛋白N端、Met-N端或C端的FLAG®肽。
比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。
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