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P4716

果胶酶 来源于黑曲霉

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BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

别名:

多聚半乳糖醛酸酶 溶液 来源于黑曲霉, 聚-（1,4-& # 945;-D·-半乳糖醛酸）聚糖水解酶

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关于此项目

化学文摘社编号:
9032-75-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-885-6
MDL number:
MFCD00131809
EC 号:
3.2.1.15 (BRENDA, IUBMB)
Specific activity:
≥5 units/mg protein (Lowry)
Biological source:
Aspergillus niger
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biological source

Aspergillus niger

Quality Level

200

product line

BioReagent

form

aqueous glycerol solution

specific activity

≥5 units/mg protein (Lowry)

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

cell culture | plant: suitable

greener alternative category

, Enabling

storage temp.

2-8°C

General description

我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品针对纤维素乙醇研究进行了优化，可提高能效并防止浪费。有关详细信息，请参阅《biofiles》中的文章和《替代能源研究用酶》

Application

黑曲霉来源果胶酶可用于：
  • 原生质体释放
  • 原生质体酶混合物，进行酶解
  • 糖的部分糖化
  • 研究其在植物病原菌入侵植物组织、农产品变质、各种食品加工和植物生物技术应用中的作用

用于植物原生质体制备，在细胞器分离前消化细胞壁。

Biochem/physiol Actions

从黑曲霉中选定菌株生成的果胶酶制剂：主要含有果胶转移酶、多聚半乳糖醛酸酶和果胶酯酶以及少量的半纤维素酶和纤维素酶。果胶酶水解果胶，果胶是细胞壁的一个组成部分。它们可能攻击甲基酯化果胶或去酯化果胶。它是果胶酶活性的来源，还含有纤维素酶和半纤维素酶活性。果胶酶催化果胶和其他半乳糖醛酸中的a-（1-4）-Dgalactosiduronic键的随机水解。

Physical form

40%甘油溶液

Other Notes

在25°C条件下，当pH为4.0时，一个单位每分钟将释放从多聚半乳糖醛酸中得到的1.0 μ摩尔的半乳糖醛酸。
有关复合碳水化合物分析 酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000460441
SLBT6394
SLBH8994V
SLBG1533V
SLBB1928V

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实验方案

果胶酶的酶促检测

在测定果胶酶活性时，使用了一种滴定终止反应法进行测定。在25 °C、pH 4.0条件下，一个单位的果胶酶每小时可从多聚半乳糖醛酸释放1 μmol 的半乳糖醛酸。

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

商品

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Karyotypes of some species of Castanopsis, Lithocarpus and Quercus (Fagaceae) from Khun Mae Kuang Forest in Chiang Mai province, northern Thailand
Chokchaichamnankit P, et al.
Thai Forest Bulletin , 38-44 (2007)
Tony Heitkam et al.
The Plant journal : for cell and molecular biology, 103(1), 32-52 (2020-01-26)
If two related plant species hybridize, their genomes may be combined and duplicated within a single nucleus, thereby forming an allotetraploid. How the emerging plant balances two co-evolved genomes is still a matter of ongoing research. Here, we focus on
Trude Schwarzacher
Methods in molecular biology (Clifton, N.J.), 1370, 87-103 (2015-12-15)
Good preparations are essential for informative analysis of both somatic and meiotic chromosomes, cytogenetics, and cell divisions. Fluorescent chromosome staining allows even small chromosomes to be visualized and counted, showing their morphology. Aneuploidies and polyploidies can be established for species
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