活细胞成像试剂

实时活细胞成像使科学家能够监测动态的细胞事件，而传统的终点检测技术（如固定细胞成像和PCR）仅能捕捉某个静态时刻，因此无法观察到这些动态过程。我们的活细胞成像试剂产品组合涵盖用于细胞追踪的创新荧光细胞标记技术、荧光慢病毒生物传感器，以及用于实时分析细胞事件的传统荧光染料和探针。这些试剂非常适合配合CellASIC® ONIX2微流控活细胞成像系统等实时孵育和成像系统来捕捉动态细胞事件，但也可以像传统的荧光细胞培养试剂一样，配合标准或共聚焦荧光显微镜使用。

产品表下方继续介绍精选产品，包括PKH和CellVue®荧光细胞连接子标记、LentiBrite™荧光慢病毒生物传感器、BioTracker®活细胞荧光染料和染色剂等。

PKH 和 CellVue® 荧光细胞连接剂标记

PKH 和 CellVue® 荧光细胞连接剂试剂盒可对活细胞进行荧光膜标记，适用于长期活细胞成像实验。

PKH 和 CellVue® 染料的主要特性包括：

在体内活细胞上可保持稳定长达 100 天
对真核细胞、细菌细胞和寄生虫均安全
无显著的染料渗漏或细胞间转移
信号均匀且可重复
光谱不同的染料可实现多色分析
与其他荧光标记物兼容
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LentiBrite™ 荧光慢病毒生物传感器

LentiBrite™ 荧光慢病毒生物传感器是一套预包装的慢病毒颗粒，其中编码与自噬、凋亡和细胞结构相关的荧光蛋白，用于深入了解活细胞中这些过程。

慢病毒生物传感器特点：

预包装、即用型，标记有单体GFP和RFP
每支试管最低滴度（≥3 × 10⁸ IFU/mL）
转染效率优于基于化学试剂的方法
适用于增殖细胞、非增殖细胞以及原代细胞或干细胞等难以转染的细胞
不干扰细胞功能

BioTracker® 活细胞荧光染料和染色剂

BioTracker® 活细胞染料是一类可穿透细胞膜的染料，适用于活细胞应用，例如细胞器标记、凋亡检测、细胞存活率和细胞健康状况分析、缺氧监测、活性氧追踪、钙离子指示功能，以及神经细胞和干细胞（SCR150、SCT029、SCT046）培养。

LuminiCell Tracker™ 荧光纳米颗粒

LuminiCell Tracker™ 荧光纳米颗粒为长期细胞追踪实验提供了一种有效、简单且无毒的试剂。  基于聚集体诱导发光（AIE）技术，这些细胞追踪染料已被封装到生物相容性、可穿透细胞的荧光纳米颗粒（AIE Dot）中，可为长期生物成像应用提供增强的光稳定性、超亮荧光以及低细胞毒性。

更亮：比许多其他细胞标记技术亮10倍
光稳定性：荧光寿命延长3倍，无信号淬灭
生物相容性：专为生物应用开发的无毒有机纳米颗粒
快速操作流程：操作简便，四小时内即可完成细胞标记

我们的最新款 AIE 细胞追踪剂（SCT017）提供多种荧光发射波长以优化多路复用，其波长位于关键的近红外（NIR）范围内，可增强应用灵活性并拓展多路复用潜力。

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利用CellASIC® ONIX2微流控系统对癌细胞缺氧现象进行活细胞成像

A431 epidermal carcinoma cells (85090402) were incubated 16 hours overnight with 3µM BioTracker 520 Green Hypoxia Dye (SCT033) in complete DMEM by gravity perfusion at 37°C under normoxic conditions (20% O2) using the CellASIC® ONIX2 System. After overnight incubation, cells were perfused with complete DMEM media without dye at 1 psi under hypoxic conditions (0% O2) for 16 hours at 37°C. Fluorescent images were captured over 16 hours using 465 nm excitation, and a 525 nm emission filter. The BioTracker 520 Green Hypoxia Dye increases in fluorescence intensity with decreasing oxygen levels (increased hypoxic conditions).

Live Cell Imaging of p62 using LentiBrite™ Fluorescent Biosensors

Human mesenchymal stem cells (MSCs), were plated in a chamber slide and transduced with LentiBrite™ RFP-p62 lentiviral particles at an MOI of 40 for 24 hours. After media replacement and 24 hours further incubation, cells were incubated for 4 hours in EBSS containing a lysosome inhibitor, to induce autophagy and inhibit lysosomal degradation of autophagosomes.

Live Cell Imaging of β-Actin Cytoskeleton Proteins using LentiBrite™ Fluorescent Biosensors

U2OS cells were plated in a chamber slide and transduced with LentiBrite™ GFP-β-actin lentiviral particles at an MOI of 20 for 24 hours. After media replacement and an additional 48 hours of further incubation, the cells were treated with 2 µM cytochalasin-D for another 2 hours, to induce actin depolymerization. The GFP- β -actin displays a filamentous distribution in the untreated cells, and upon actin depolymerization, adopts a punctate distribution. LentiBrite™ Lentiviral Biosensors are a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.

Live Cell Imaging of Autophagy using LentiBrite™ Fluorescent Biosensors

HT-1080 cells were plated in a chamber slide and transduced with LentiBrite™ GFP-LC3 lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were incubated for 4 hours in EBSS containing a lysosome inhibitor, to induce autophagy and inhibit lysosomal degradation of autophagosomes. LentiBrite™ Lentiviral Biosensors are a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.

Live Cell Imaging of Apoptosis in Immune Cells using the CellASIC® ONIX2 Microfluidic System

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.