HEPES solution

Name: HEPES solution
Brand: SIGMA

1 M in H₂O, liquid

Synonym(s):

HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

CAS Number:

7365-45-9

UNSPSC Code:

12161700

eCl@ss:

32129211

PubChem Substance ID:

57652951

NACRES:

NA.25

MDL number:

MFCD00006158

Product Name

HEPES buffer solution, 1 M in H₂O

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

form

liquid

concentration

1 M in H₂O
238 g/L HEPES in H₂O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5±0.5

useful pH range

6.8-8.2

density

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

Quality Level

100

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Related Categories

Biochemicals

Biological Buffers

HEPES Buffer

Application

HEPES has been used:

As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
As a component of dissection buffer for culturing rat cortical neurons
Along with MgCl₂, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
As a component of Danieu′s aquarium water used in agarose gel preparation

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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