B5655
BCIP®/NBT
SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet
Synonym(s):
BCIP/NBT Substrate, BCIP®/NBT Alkaline Phosphatase Substrate
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Product Name
SIGMAFAST™ BCIP®/NBT, tablet
form
tablet
Quality Level
solubility
water: 10 mL, clear, yellow
storage temp.
−20°C
Application
SIGMAFAST BCIP®/NBT has been used as a substrate
- for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
- for alkaline phosphatase in the histochemical staining of primary osteoblasts
- for alkaline phosphatase staining in bone marrow stromal cells
Preparation Note
Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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Storage Class
11 - Combustible Solids
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MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
Aneela Yasmeen et al.
Molecular biotechnology, 58(12), 807-820 (2016-10-21)
The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct