I1284
Isopropyl β-D-thiogalactopyranoside solution
ReadyMade IPTG solution for Blue-white screening
Synonym(s):
IPTG solution
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About This Item
Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
Molecular Weight:
238.30
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4631
Product Name
Isopropyl β-D-thiogalactopyranoside solution, ReadyMade IPTG solution for Blue-white screening
Quality Level
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
SMILES string
CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
InChI key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
grade
Molecular Biology
description
non-ionic
form
liquid
mol wt
238.3
shipped in
dry ice
storage temp.
−20°C
Application
Isopropyl β-D-thiogalactopyranoside solution is:
- used as an inducer for the expression of single domain antibodies(sdAbs) in E coli and bioactive peptides
- used in its half-life and decay studies
- suitable for induction of recombinant protein expression in systems controlled by the lac repressor
Biochem/physiol Actions
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.
General description
Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots
Sukhanova A, et al.
Protocol Exchange, 10 (2012)
Random sequences are an abundant source of bioactive RNAs or peptides
Neme R, et al.
Nature ecology & evolution, 1(6), 0127-0127 (2017)
Half-life measurements of chemical inducers for recombinant gene expression
Pasotti L, et al.
Journal of Biological Engineering, 8(1), 5-5 (2014)
L H Hansen et al.
Current microbiology, 36(6), 341-347 (1998-06-03)
The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with
Therese Wohlschlager et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(27), E2787-E2796 (2014-06-01)
Effector proteins of innate immune systems recognize specific non-self epitopes. Tectonins are a family of β-propeller lectins conserved from bacteria to mammals that have been shown to bind bacterial lipopolysaccharide (LPS). We present experimental evidence that two Tectonins of fungal
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