V4505
Anti-Vinculin Antibody
mouse monoclonal, VIN-11-5
Synonym(s):
Vinculin Antibody Sigma, Anti-VCL
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About This Item
Product Name
Monoclonal Anti-Vinculin antibody produced in mouse, clone VIN-11-5, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
VIN-11-5, monoclonal
mol wt
antigen 116 kDa
species reactivity
bovine, human, chicken, mouse
technique(s)
indirect immunofluorescence: 1:50 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... VCL(7414)
mouse ... Vcl(22330)
Application
Monoclonal Anti-Vinculin antibody produced in mouse has been used in:
- western blotting.
- immunofluorescence.
- immunocytochemistry.
Biochem/physiol Actions
The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.
Vinculin (VCL) is a cytoskeletal protein, which plays a vital role in regulation of cell-to-cell and cell to matrix junctions. VCL facilitates normal embryonic development and cardiac function. Aberration or mutation of the gene leads to cardiomyopathy. VCL also plays a crucial role in cell migration and morphogenesis. VCL might facilitate the binding of integrins and cadherins to the actin cytoskeleton. Vinculin plays an important role in integrin αIIbβ3 signaling and function, which is required for platelet aggregation.
Vinculin is suggested to be a possible link between ends of the bundles of actin filaments and the plasma membrane. It has also been proposed that vinculin may be involved in the transmembrane induction of actin bundle formation.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The vinculin (VCL) gene containing 22 exons is mapped to human chromosome 10q22.1-q23. The gene codes for a 117kDa cytoskeletal protein present in adherens type cell junctions. VCL is a ubiquitously expressed cell-adhesion protein. The protein is characterized with a globular head and a tail domain, which are interconnected by a short proline-rich sequence, an intramolecular interaction between the head and tail conceal many ligand-binding sites in the protein.
Immunogen
smooth muscle vinculin from chicken gizzard.
Other Notes
This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200729 Anti-Vinculin antibody, Mouse monoclonal
clone VIN-11-5, purified from hybridoma cell culture
SAB4200729 Anti-Vinculin antibody, Mouse monoclonal
clone VIN-11-5, purified from hybridoma cell culture
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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E P Moiseyeva et al.
The Journal of biological chemistry, 268(6), 4318-4325 (1993-02-25)
The human vinculin gene contains 22 exons ranging in size from 71 base pairs (bp) to 303 bp (average 155 bp) with the exception of exon 22 which contains 144 bp of coding sequence and 1848 bp of 3'-untranslated sequence
Three-dimensional structure of vinculin bound to actin filaments
Janssen MEW, et al.
Molecular Cell, 21(2), 271-281 (2006)
W Xu et al.
Journal of cell science, 111 ( Pt 11), 1535-1544 (1998-05-15)
Vinculin plays a role in signaling between integrins and the actin cytoskeleton. We reported earlier that F9-derived cells lacking vinculin are less spread, less adhesive, and move two times faster than wild-type F9 cells. Expression of intact vinculin in null
Liyong Zhang et al.
Nature communications, 5, 3430-3430 (2014-03-13)
The HECT E3 ubiquitin ligase HACE1 is a tumour suppressor known to regulate Rac1 activity under stress conditions. HACE1 is increased in the serum of patients with heart failure. Here we show that HACE1 protects the heart under pressure stress
Meng Chen et al.
The Journal of biological chemistry, 288(48), 34647-34657 (2013-10-24)
The mylk1 gene encodes a 220-kDa nonmuscle myosin light chain kinase (MLCK), a 130-kDa smooth muscle MLCK (smMLCK), as well as the non-catalytic product telokin. Together, these proteins play critical roles in regulating smooth muscle contractility. Changes in their expression
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