Anti-phospho-PAK1 (Ser199/Ser204) Antibody

65 kDa

p21-activated kinases (PAK) are Ser/Thr kinases, which are activated by, and are effectors of Rho family of GTPases. As such, they are implicated in Actin polymerization and activation of the stress-activated kinase cascades. PAK1 is phosphorylated by cdk5, resulting in its inhibition. PAK2 can be activated by Caspase-cleavage in addition to p21-binding, implicating it in cytoskeletal changes during apoptosis. PAK3 is expressed at high levels in post-mitotic neurons of the developing post-natal cerebral cortex and hippocampus, and is the mutant gene thought responsible for non-syndromic X-linked mental retardation.

Amino Acids encompassing and including phosphorylated Ser199 and Ser204 of human PAK1. Sequence is highly homologous (greater than 90%) to PAK2 and PAK3.

Epitope: Ser199/Ser204 (Central)

Detect phospho-PAK1 (Ser199/Ser204) using this Anti-phospho-PAK1 (Ser199/Ser204) Antibody validated for use in WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Recognizes PAK1 phosphorylated on Ser199 and Ser204 (human)/Ser198 and Ser203 (mouse). Immunogen sequence is higly homolgous (greater than 90%) with PAK2 and PAK3.

Affinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol

Double affinity purification over both non-phospho-peptide column followed by phopshorylated peptide column.

Stable for 1 year at -20°C from date of receipt.

Routinely evaluated by western blot on untreated and pervanadate-treated Jurkat cell lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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