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648317

Tris, Hydrochloride, Molecular Biology Grade

Name: Tris, Hydrochloride, Molecular Biology Grade
Brand: MM

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Synonym(s):

Trizma^® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

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About This Item

Linear Formula:

NH₂C(CH₂OH)₃ · HCl

CAS Number:

1185-53-1

Molecular Weight:

157.60

NACRES:

NA.25

UNSPSC Code:

12161705

Beilstein/REAXYS Number:

3675235

MDL number:

MFCD00012590

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Properties

grade

Molecular Biology

Quality Level

100

description

RTECS - TY2900000

assay

≥99% (titration)

form

crystalline solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic)

color

white

useful pH range

7.0-9.0

pKa (25 °C)

8.1

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤0.0002%

absorption

≤0.05 at 260 nm, ≤0.05 at 280 nm

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

General description

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used biological buffer (absorbance: ≤0.05 at 260 nm and 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c