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D3252

o-Dianisidine dihydrochloride

≥95%

Synonym(s):

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Empirical Formula (Hill Notation):
C14H16N2O2 · 2HCl
CAS Number:
20325-40-0
Molecular Weight:
317.21
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24893736
EC Number:
243-737-5
Beilstein/REAXYS Number:
3917996
MDL number:
MFCD00012488
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Quality Level

200

assay

≥95%

form

powder

mp

268 °C

solubility

H2O: soluble 25 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

Application

o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.

Biochem/physiol Actions

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Physical form

crystalline

Disclaimer

Probably carcinogenic.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ0081
MKCX5906
MKCW7554
MKCW1665
MKCT8252

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Luminol-enhanced chemiluminescence induced in peripheral blood-derived human phagocytes: obligatory requirement of myeloperoxidase exocytosis by monocytes.
Albrecht D and Jungi TW
Journal of Leukocyte Biology, 54, 300-300 (1993)
Peter M Meggyesy et al.
Molecules (Basel, Switzerland), 25(21) (2020-10-31)
The therapeutic utility of the copper ionophore disulfiram was investigated in a diet-induced obesity mouse model (C57BL/6J background), both through administration in feed (0.05 to 1% (w/w)) and via oral gavage (150 mg/kg) for up to eight weeks. Mice were
Interspecies interactions determine the impact of the gut microbiota on nutrient allocation in Drosophila melanogaster.
Newell PD and Douglas AE
Applied and Environmental Microbiology, 80, 788-788 (2014)
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